4WH9
Structure of the CDC25B Phosphatase Catalytic Domain with Bound Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.660, 71.470, 73.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.390 - 1.500 |
| R-factor | 0.1273 |
| Rwork | 0.126 |
| R-free | 0.15201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a2k |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.928 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.390 | 1.400 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.098 | 0.460 |
| Number of reflections | 63905 | |
| <I/σ(I)> | 10.5 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | (NH4)2SO4, Tris, TCEP |
