4WGZ
Crystal Structure of Cytochrome c' from Alcaligenes xylosoxidans NCIMB 11015 at pH 6.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 53.189, 53.189, 181.026 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.310 - 1.110 |
R-factor | 0.14158 |
Rwork | 0.141 |
R-free | 0.16168 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e83 |
RMSD bond length | 0.030 |
RMSD bond angle | 3.094 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.100 |
Number of reflections | 62734 |
<I/σ(I)> | 36.1 |
Completeness [%] | 99.4 |
Redundancy | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6 | 293 | 12 % PEG4000, 100 mM potassium phosphate, 1 mM sodium azide |