4WGY

Crystal Structure of Cytochrome c' from Alcaligenes xylosoxidans NCIMB 11015 at pH 10.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.7500
Spacegroup name	P 65 2 2
Unit cell lengths	52.666, 52.666, 183.584
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.610 - 1.480
R-factor	0.14002
Rwork	0.138
R-free	0.17253
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e83
RMSD bond length	0.026
RMSD bond angle	2.770
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.480
Number of reflections	26188
<I/σ(I)>	64.1
Completeness [%]	99.6
Redundancy	20.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.4	293	24 % PEG4000, 100 mM CAPS, 50 mM magnesium chloride