4WFY

Coxsackievirus B3 Polymerase - F232L Mutant - AmSO4 Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-12
Detector	NOIR-1
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	74.360, 74.360, 288.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.580 - 2.060
R-factor	0.1886
Rwork	0.187
R-free	0.22010
RMSD bond length	0.007
RMSD bond angle	1.057
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	52.600
High resolution limit [Å]	2.060
Number of reflections	710311
<I/σ(I)>	18.65
Completeness [%]	99.3
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	0.085 M Tris, 1.275 M Ammonium Sulfate , 25% Glycerol