4WFT

Crystal structure of tRNA-dihydrouridine(20) synthase dsRBD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-17
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 43
Unit cell lengths	55.130, 55.130, 114.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.738 - 1.700
R-factor	0.1639
Rwork	0.163
R-free	0.18020
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	1.750
High resolution limit [Å]	1.700	1.690
Rmerge		0.336
Number of reflections	38156
<I/σ(I)>	22.06	4.16
Completeness [%]	99.7	97.65
Redundancy	4.51	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	Protein 20 mg/mL precipitant: HEPES 100 mM pH 6.5, sodium acetate 100 mM, PEG 2000 MME 30%
1	VAPOR DIFFUSION, HANGING DROP		292	Protein 20 mg/mL precipitant: HEPES 100 mM pH 6.5, sodium acetate 100 mM, PEG 2000 MME 30%