4WFS
Crystal Structure of tRNA-dihydrouridine(20) synthase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.570, 85.260, 144.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.640 - 2.680 |
| R-factor | 0.1985 |
| Rwork | 0.197 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b0p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.640 | 2.770 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Number of reflections | 12686 | |
| <I/σ(I)> | 19.87 | 2.69 |
| Completeness [%] | 99.1 | 91.76 |
| Redundancy | 7.2 | 7.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | Protein 8 mg/mL in Tris 25 mM pH 8.0 NaCl 150 mM Precipitant: Ammonium sulfate 2.2 M, isopropanol 2% v/v |
