4WFE
Human TRAAK K+ channel in a K+ bound conductive conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.795, 138.522, 96.843 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 48.200 - 2.500 |
| R-factor | 0.20086 |
| Rwork | 0.199 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i9w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 69456 | |
| <I/σ(I)> | 22.6 | 0.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 50 mM Tris pH 8.8, 200 mM CaCl2, 27-30% (vol/vol) PEG400, 4% (vol/vol) polypropylene glycol |
