4WFB

The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with BC-3205

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 65 2 2
Unit cell lengths	280.918, 280.918, 875.585
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.539 - 3.430
R-factor	0.2061
Rwork	0.204
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wce
RMSD bond length	0.006
RMSD bond angle	1.099
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.470
High resolution limit [Å]	3.430	10.620	3.430
Rmerge	0.152	0.098	0.744
Total number of observations	2109298
Number of reflections	253918
<I/σ(I)>	6
Completeness [%]	92.1	99.7	78.9
Redundancy	8.3	15.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Hepes, KCl, MgCl2, NH4Cl, MPD, EtOH, Spermidine