4WF2

Structure of E. coli BirA G142A bound to biotinol-5'-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-05
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	46.239, 46.239, 157.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.850 - 2.310
R-factor	0.1744
Rwork	0.172
R-free	0.21830
RMSD bond length	0.004
RMSD bond angle	0.802
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.310
Number of reflections	14338
<I/σ(I)>	11.68
Completeness [%]	99.4
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M TrisHCl, pH 8.0 and 12.5% (w/v) PEG 8K (Hampton) at 20 deg C