4WF0

Crystal Structure of iLID - an Improved Light-Inducible Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-31
Detector	RAYONIX MX300HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	62.156, 70.339, 80.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.078 - 1.950
R-factor	0.2317
Rwork	0.231
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v0u
RMSD bond length	0.013
RMSD bond angle	1.257
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	80.380
High resolution limit [Å]	1.950
Rmerge	0.088
Number of reflections	26353
<I/σ(I)>	9.63
Completeness [%]	99.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	Grown in dark for 3 days. Conditions: 100mM TRIS:HCl pH 8.5, 800mM Lithium Chloride, 32% PEG 4000