4WEU
Co-complex structure of the F4 fimbrial adhesin FaeG variant ad with llama single domain antibody V3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 79.800, 95.200, 113.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.540 - 2.610 |
| R-factor | 0.20643 |
| Rwork | 0.204 |
| R-free | 0.24296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hlr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.601 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.520 | 2.670 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 26553 | |
| <I/σ(I)> | 16.1 | 1.1 |
| Completeness [%] | 98.3 | 79.6 |
| Redundancy | 10.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.12 M Ethylene Glycols (Di-Ethyleneglycol; Tri-Ethyleneglycol; TetraEthyleneglycol; Penta-Ethyleneglycol); 0.1 M Tris (base); Bicine pH 8.5; 37.50% % v/v MPD (racemic); PEG 1K; PEG 3350 |
