4WEM
Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 145.483, 145.483, 38.847 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.620 - 1.550 |
| R-factor | 0.17008 |
| Rwork | 0.169 |
| R-free | 0.19326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hlr |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.715 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 68481 | |
| <I/σ(I)> | 24.8 | 4.2 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 11.2 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Potassium bromide, 0.1 M Sodium acetate pH 5.5, 15 % w/v PEG 4000 |
