4WE2
Donor strand complemented FaeG of F4ab fimbriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.639, 49.026, 54.468 |
| Unit cell angles | 90.00, 109.21, 90.00 |
Refinement procedure
| Resolution | 51.430 - 1.500 |
| R-factor | 0.17704 |
| Rwork | 0.175 |
| R-free | 0.21396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hlr |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.058 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.430 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 41977 | |
| <I/σ(I)> | 13.8 | 2.8 |
| Completeness [%] | 97.9 | 89.1 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18 mM Na/K-phosphate, 18% PEG 3350 |
