4WD9
Crystal structure of tRNA-dependent lantibiotic dehydratase NisB in complex with NisA leader peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-03-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.500, 107.280, 135.730 |
| Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
| Resolution | 34.459 - 2.900 |
| R-factor | 0.1974 |
| Rwork | 0.194 |
| R-free | 0.25690 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.500 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 58894 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 99.6 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 282 | 100mM Bicine pH 8.5, 15%-25% PEG 6000 |
