4WCY
Fab fragment of mouse AZ130 monoclonal antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.157, 68.825, 113.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 2.000 |
| R-factor | 0.17465 |
| Rwork | 0.172 |
| R-free | 0.22354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k26 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | |
| Number of reflections | 34652 | |
| <I/σ(I)> | 13.09 | |
| Completeness [%] | 98.8 | 91.6 |
| Redundancy | 4.83 | 4.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Ammonium sulfate 0.2 M, BIS-TRIS 0.1 M, Polyethylene glycol 3,350 25 %w/v |
