4WCL

Crystal Structure of product bound Cell Shape Determinant protein Csd4 from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	53.030, 66.770, 144.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.758 - 1.850
R-factor	0.1709
Rwork	0.170
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.089
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.760		1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.053	0.021	0.388
Rmeas	0.062	0.024	0.455
Total number of observations	315590
Number of reflections	84317	904	6254
<I/σ(I)>	15.53	42.02	3.13
Completeness [%]	99.6	95.4	99.2
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	16-20% PEG 3350, 0-0.1 mM Tris and 0.3-0.4 M NaI