4WBZ

tRNA-processing enzyme (apo form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-12-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	58.600, 109.660, 85.270
Unit cell angles	90.00, 110.09, 90.00

Refinement procedure

Resolution	19.867 - 3.300
R-factor	0.2036
Rwork	0.203
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wby
RMSD bond length	0.002
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge	0.170	0.026	1.558
Rmeas	0.195	0.032	1.790
Total number of observations	64039
Number of reflections	15192	158	1036
<I/σ(I)>	9.27	40.07	1.07
Completeness [%]	99.0	88.3	92
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEGMME550, NaCl, Tris-Cl