4WBA

Q/E mutant SA11 NSP4_CCD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	39.857, 53.469, 52.543
Unit cell angles	90.00, 95.84, 90.00

Refinement procedure

Resolution	28.240 - 1.799
R-factor	0.1899
Rwork	0.187
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o1k
RMSD bond length	0.006
RMSD bond angle	0.923
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.240
High resolution limit [Å]	1.799
Rmerge	0.060
Number of reflections	20053
<I/σ(I)>	15.21
Completeness [%]	99.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	296	10%w/v PEG 8000, 0.2M NaCl, 0.1M Na/K PO4 (pH 6.2)