4WB8

Crystal structure of human cAMP-dependent protein kinase A (catalytic alpha subunit), exon 1 deletion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-25
Detector	MAR CCD 165 mm
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	72.808, 75.864, 80.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.274 - 1.550
R-factor	0.1642
Rwork	0.163
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gfc
RMSD bond length	0.011
RMSD bond angle	1.335
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.050	0.027	0.667
Rmeas	0.055	0.029	0.745
Rpim	0.023	0.012	0.325
Total number of observations	363415
Number of reflections	65054
<I/σ(I)>	11.7
Completeness [%]	99.0	98.3	91.1
Redundancy	5.6	5.5	4.6
CC(1/2)		0.998	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	10% PEG 8000, 0.1M HEPES pH 7.5