4WB0

Crystal structure of the broad specificity aminotransferase from Leishmania mexicana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	86.418, 90.564, 142.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.381 - 1.910
R-factor	0.1783
Rwork	0.177
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cst
RMSD bond length	0.016
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.400	1.940
High resolution limit [Å]	1.910	1.910
Rmerge	0.055	0.629
Number of reflections	86628
<I/σ(I)>	17.4	2.1
Completeness [%]	99.3	95.8
Redundancy	4.5	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		284	1 M tri-sodium citrate, 0.1 M sodium cacodylate