4WAS
STRUCTURE OF THE ETR1P/NADP/CROTONYL-COA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-11-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.920, 106.140, 94.410 |
| Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
| Resolution | 37.815 - 1.700 |
| R-factor | 0.1882 |
| Rwork | 0.188 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n9g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.178 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 156706 | |
| <I/σ(I)> | 10.6 | 1 |
| Completeness [%] | 99.0 | 98.9 |
| Redundancy | 3.1 | 3.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 292 | 2M ammonium sulfate, 0.1M ADA/NaOH pH 7 |
