4WA1
The crystal structure of hemagglutinin from a H3N8 influenza virus isolated from New England harbor seals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.061, 103.155, 110.864 |
| Unit cell angles | 89.95, 90.00, 90.28 |
Refinement procedure
| Resolution | 46.779 - 1.898 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yp2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.134 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.898 |
| Number of reflections | 272212 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 97.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 0.2 M ammonium iodide, 20% PEG3350 |
