4W9Z
Crystal structure of the periplasmic domain of subunit II of cytochrome oxidase (CoxB) of Bradyrhizobium japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-08-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.431, 38.612, 59.644 |
| Unit cell angles | 90.00, 97.63, 90.00 |
Refinement procedure
| Resolution | 59.120 - 1.300 |
| R-factor | 0.1102 |
| Rwork | 0.109 |
| R-free | 0.13360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4txv |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.270 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 1.330 | ||
| High resolution limit [Å] | 1.300 | 5.810 | 1.300 |
| Rmerge | 0.048 | 0.033 | 0.247 |
| Rmeas | 0.053 | 0.035 | 0.272 |
| Total number of observations | 231266 | ||
| Number of reflections | 35883 | 437 | 2652 |
| <I/σ(I)> | 20.13 | 53.23 | 5.25 |
| Completeness [%] | 99.2 | 98.9 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M Potassium bromide, 0.1M Tris pH 7.5, 10% PEG1000, 10% PEG8000 |
