4W8P
Crystal structure of RIAM TBS1 in complex with talin R7R8 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.722, 105.884, 49.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.790 - 1.500 |
| R-factor | 0.22363 |
| Rwork | 0.223 |
| R-free | 0.24192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.934 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.558 | |
| Number of reflections | 49933 | |
| <I/σ(I)> | 47.3 | 2.9 |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 5.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol |
