4W7Y
Dimeric BAP29 vDED with disulfide bonds in crystal contacts
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97800 |
Spacegroup name | P 65 |
Unit cell lengths | 118.870, 118.870, 30.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.315 - 2.500 |
R-factor | 0.1973 |
Rwork | 0.195 |
R-free | 0.23520 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.275 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.310 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 16720 | |
<I/σ(I)> | 17.09 | 2.93 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 292.15 | Grown in 34% 2-methyl-2,4-pentanediol (MPD), 100 mM Na acetate pH 4.6 and 20 mM CaCl2 using SeMet labeled protein purified in 20 mM Tris pH 8, 150 mM NaCl and 2 mM tris(2-carboxyethyl)phosphine (TECEP) and concentrated to ~13 mg/mL. |