4W7G

Crystal Structure of the Dynein Light Intermediate Chain's Conserved Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12
Spacegroup name	C 2 2 21
Unit cell lengths	59.370, 138.670, 112.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.108 - 2.100
R-factor	0.1766
Rwork	0.174
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.232
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge		1.350
Number of reflections	27518
<I/σ(I)>	12.3	1.7
Completeness [%]	99.9	99.7
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES, and 20% (w/v) PEG 10000