4W6H

Crystal Structure of Full-Length Split GFP Mutant D190C Disulfide Dimer, P 65 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0717
Spacegroup name	P 65
Unit cell lengths	95.520, 95.520, 132.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.720 - 1.953
R-factor	0.169
Rwork	0.166
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.017
RMSD bond angle	1.613
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.720
High resolution limit [Å]	1.953
Number of reflections	49488
<I/σ(I)>	16.42
Completeness [%]	99.8
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M SPG Buffer pH8.0,25% PEG-1500