4W6G

Crystal Structure of Full-Length Split GFP Mutant D190C Disulfide Dimer, P 61 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 61
Unit cell lengths	93.380, 93.380, 132.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.090 - 3.024
R-factor	0.251
Rwork	0.248
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	69.090
High resolution limit [Å]	3.024
Number of reflections	12859
<I/σ(I)>	16.73
Completeness [%]	99.7
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	20% 1,4-Butanediol, 0.1M Acetate pH 4.5