4W69
Crystal Structure of Full-Length Split GFP Mutant Q157C Disulfide Dimer, P 43 21 2 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0717 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.760, 133.760, 88.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.580 - 3.975 |
| R-factor | 0.31 |
| Rwork | 0.307 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.912 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.580 | 4.120 |
| High resolution limit [Å] | 3.975 | 3.975 |
| Rmerge | 1.660 | |
| Number of reflections | 7344 | |
| <I/σ(I)> | 16.94 | 2.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 15 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.4M MgFormate, 0.1M Acetate pH 4.6, 2%w/v benzamidine |
