4W64
Hcp1 protein from Acinetobacter baumannii AB0057
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 6 |
Unit cell lengths | 87.001, 87.001, 128.809 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.956 - 1.550 |
R-factor | 0.1695 |
Rwork | 0.168 |
R-free | 0.19770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y12 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.088 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.960 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 79939 | |
<I/σ(I)> | 20.8 | 1.8 |
Completeness [%] | 99.8 | 98.06 |
Redundancy | 10.8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.0 M Ammonium Sulfate and 0.1 M Hepes |