4W60

The structure of Vaccina virus H7 protein displays A Novel Phosphoinositide binding fold required for membrane biogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	118.714, 68.048, 81.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.040 - 2.700
R-factor	0.26
Rwork	0.256
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.170
Refinement software	PHENIX ((PHENIX.REFINE: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	18495
<I/σ(I)>	19.5
Completeness [%]	98.4
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.08M Bis-Tris phosphate/citric acid, pH8.8, 18% (w/v) PEG 3350