4W5W

Rubisco activase from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 65
Unit cell lengths	83.850, 83.850, 105.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.930 - 2.900
R-factor	0.1983
Rwork	0.196
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t15
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS (March 30, 2013)
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.5.2)
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.930	41.930	3.080
High resolution limit [Å]	2.900	8.700	2.900
Rmerge	0.057	0.014	0.508
Rpim	0.031	0.008	0.275
Total number of observations	41585	1505	6765
Number of reflections	9423
<I/σ(I)>	19.5	70.7	3.1
Completeness [%]	99.9	99.1	99.9
Redundancy	4.4	4.1	4.5
CC(1/2)	0.999	1.000	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein:Reservoir 1:1 Protein sample:8 mg ml-1 protein, 20 mM Bis-Tris propane pH 8, 50 mM KCl, 10% glycerol, 5 mM TCEP Reservoir solution: 100 mM Tris pH 7, 0.15 M AmSO4, 13% PEG 3350, 5 mM TCEP