4W4N
Crystal structure of human Fc at 1.80 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.710, 79.290, 138.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.188 - 1.800 |
| R-factor | 0.20132 |
| Rwork | 0.200 |
| R-free | 0.24436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.125 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.787 |
| Number of reflections | 51158 | |
| <I/σ(I)> | 18.4 | 2.4 |
| Completeness [%] | 99.1 | 97.3 |
| Redundancy | 6.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.2M calcium acetate, 0.1M sodium cacodylate, 19% PEG 8000 |
