4W2O
Anti-Marburgvirus Nucleoprotein Single Domain Antibody B Complexed with Nucleoprotein C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-23 |
Detector | NOIR-1 |
Wavelength(s) | 0.97626 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.998, 108.657, 141.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.328 - 3.200 |
R-factor | 0.2325 |
Rwork | 0.227 |
R-free | 0.28460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6app |
RMSD bond length | 0.003 |
RMSD bond angle | 0.564 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rpim | 0.081 | 0.297 |
Number of reflections | 15378 | 2189 |
<I/σ(I)> | 10.6 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% polyethylene glycol 4000, 0.16M ammonium sulfate, 20% glycerol, 0.08M sodium acetate pH 4.6 |