4W29
70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Collection date | 2013-10-12 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 306.919, 677.002, 356.780 |
| Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
| Resolution | 49.980 - 3.800 |
| R-factor | 0.2964 |
| Rwork | 0.295 |
| R-free | 0.33060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3F1E 3F1F |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 357.000 |
| High resolution limit [Å] | 3.800 |
| Number of reflections | 482241 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
