4V9J

70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03322
Spacegroup name	C 1 2 1
Unit cell lengths	302.391, 683.918, 356.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.860
R-factor	0.264
Rwork	0.264
R-free	0.31660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3F1E 3F1F 2WRI AND 2WRJ
RMSD bond length	0.022
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.860
Number of reflections	462446
<I/σ(I)>	5.1
Completeness [%]	96.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
1				2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate