4V9E
Crystal Structure of Rift Valley Fever Virus Nucleocapsid Protein Hexamer Bound to Single-stranded RNA.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 |
Unit cell lengths | 91.660, 173.330, 172.900 |
Unit cell angles | 119.95, 99.34, 90.12 |
Refinement procedure
Resolution | 57.420 - 3.400 |
R-factor | 0.2254 |
Rwork | 0.225 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lyf |
RMSD bond length | 0.008 |
RMSD bond angle | 0.850 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 149.472 | 57.417 | 3.580 |
High resolution limit [Å] | 3.400 | 10.750 | 3.400 |
Rmerge | 0.050 | 0.513 | |
Total number of observations | 7343 | 31220 | |
Number of reflections | 117933 | ||
<I/σ(I)> | 4 | 3.5 | 1.3 |
Completeness [%] | 94.7 | 95.6 | 92.3 |
Redundancy | 1.9 | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% PEG3350, 350 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |