4V9D
Structures of the bacterial ribosome in classical and hybrid states of tRNA binding
This is a non-PDB format compatible entry.
Replaces: 3R8NReplaces: 3R8OExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.670, 438.070, 613.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB ENTRIES 3I1M 3I1N 3I1O 3I1P |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.160 | |
| High resolution limit [Å] | 3.000 | 9.500 | 3.000 |
| Rmerge | 0.194 | 0.077 | 0.816 |
| Number of reflections | 938380 | 36685 | 70192 |
| <I/σ(I)> | 7.38 | 27.85 | 1.18 |
| Completeness [%] | 83.5 | 99.5 | 43.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | PEG8000, MPD, pH 6.5, microbatch, temperature 291K |
| 1 | MICROBATCH | 6.5 | 291 | PEG8000, MPD, pH 6.5, microbatch, temperature 291K |
| 1 | MICROBATCH | 6.5 | 291 | PEG8000, MPD, pH 6.5, microbatch, temperature 291K |
