4V99
The Crystallographic Structure of Panicum Mosaic Virus
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 411.740, 403.900, 412.460 |
Unit cell angles | 90.00, 89.65, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.900 |
R-factor | 0.255 |
Rwork | 0.251 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c8n |
RMSD bond length | 0.009 |
RMSD bond angle | 1.410 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2L) |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.010 | 2.810 |
High resolution limit [Å] | 2.710 | 5.840 | 2.710 |
Rmerge | 0.136 | 0.065 | 0.346 |
Total number of observations | 1234096 | 39843 | |
Number of reflections | 2279719 | ||
<I/σ(I)> | 6.8 | 13.3 | 2.5 |
Completeness [%] | 63.0 | 99.6 | 4.9 |
Redundancy | 3.14 | 3.34 | 2.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 10% PEG400, 0.1 M citric acid, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |