4V96
The structure of a 1.8 MDa viral genome injection device suggests alternative infection mechanisms
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.8856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 163.530, 287.360, 323.140 |
Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
Resolution | 35.970 - 3.800 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.26410 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 4.000 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 284699 | |
<I/σ(I)> | 8.72 | 1.28 |
Completeness [%] | 99.2 | 99.1 |
Redundancy | 3.4 | 3.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 0.08M Trizma pH8.0, 0.1M KCl, 15% PEG2000MME, VAPOR DIFFUSION, temperature 293K |