4V8U
Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 291.357, 269.425, 401.947 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 49.750 - 3.700 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.200 | 1.080 |
| Number of reflections | 657561 | |
| <I/σ(I)> | 8.2 | 1.64 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4 |
