4V8Q
Complex of SmpB, a tmRNA fragment and EF-Tu-GDP-Kirromycin with the 70S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-27 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 202.170, 290.760, 250.650 |
Unit cell angles | 90.00, 97.63, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.100 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2Y11 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.410 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.240 | 0.700 |
Number of reflections | 503945 | |
<I/σ(I)> | 8.8 | 1.48 |
Completeness [%] | 97.9 | 92.6 |
Redundancy | 8.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 100 MM MES PH 6.5, 20 MM KCL, 8% (W/V) PEG20K |