4V85

Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-02
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.03318
Spacegroup name	P 21 21 21
Unit cell lengths	257.600, 312.900, 328.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.200
R-factor	0.22
Rwork	0.210
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.060
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	433307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 295K