4V7T

Crystal structure of the E. coli ribosome bound to chloramphenicol.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1158
Spacegroup name	P 21 21 21
Unit cell lengths	211.456, 434.084, 621.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.146 - 3.194
R-factor	0.1919
Rwork	0.191
R-free	0.25200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3I1M 3I1N 3I1O 3I1P
RMSD bond length	0.006
RMSD bond angle	1.427
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.420
High resolution limit [Å]	3.194	7.110	3.194
Rmerge	0.072	0.036	0.435
Number of reflections	708760
<I/σ(I)>	12.7
Completeness [%]	82.6	93	69.7
Redundancy	2	2.9	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD, pH 6.5, microbatch, temperature 291K