4V7P

Recognition of the amber stop codon by release factor RF1.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Collection date	2009-08-05
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	210.380, 452.700, 617.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.953 - 3.620
R-factor	0.26
Rwork	0.260
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3F1E and 3F1F
RMSD bond length	0.002
RMSD bond angle	0.659
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall
Low resolution limit [Å]	89.000
High resolution limit [Å]	3.514
Number of reflections	723953
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295.5	crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
1	VAPOR DIFFUSION, SITTING DROP	7	295.5	crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K