4V7G
Crystal Structure of Lumazine Synthase from Bacillus Anthracis
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9724 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 157.201, 222.237, 473.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.998 - 3.500 |
R-factor | 0.236 |
Rwork | 0.235 |
R-free | 0.28620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rvv |
RMSD bond length | 0.003 |
RMSD bond angle | 0.599 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.290 | 0.860 |
Number of reflections | 194243 | |
<I/σ(I)> | 5.2 | 2.2 |
Completeness [%] | 93.3 | 93.5 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 36% polypropilenglycol P400, 100mM Tris, 20mM DTT, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |