4V5V
Structure of respiratory syncytial virus nucleocapsid protein, P1 crystal form
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 164.347, 175.740, 241.977 |
| Unit cell angles | 90.09, 89.96, 89.92 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wj8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.120 | 0.670 |
| Number of reflections | 330486 | |
| <I/σ(I)> | 6.7 | 1.2 |
| Completeness [%] | 97.3 | 97.7 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% (V/V) MPD, 0.05 M AMMONIUM ACETATE, 0.05 M TRIS PH7.5, 0.01 M MGCL2 |
