4V4R

Crystal structure of the whole ribosomal complex.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97889
Spacegroup name	P 43 21 2
Unit cell lengths	518.993, 518.993, 365.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 5.900
R-factor	0.353
Rwork	0.351
R-free	0.37100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j5e 1nkw
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	6.200
High resolution limit [Å]	5.900	5.900
Number of reflections	149562
<I/σ(I)>	11.3	2.3
Completeness [%]	95.4	96.4
Redundancy	7.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	277	PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG20K
10	1	2	ammonium chloride
2	1	1	MES
3	1	1	magnesium acetate
4	1	1	potassium chloride
5	1	1	ammonium chloride
6	1	1	H2O
7	1	2	PEG20K
8	1	2	magnesium acetate
9	1	2	potassium chloride