4V4A
Crystal Structure of the Wild Type Ribosome from E. Coli 70S Ribosome.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-12-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0, 1.1 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 683.890, 683.890, 386.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 9.500 |
| Rwork | 0.389 |
| R-free | 0.40700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j5e 1LNR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 9.680 |
| High resolution limit [Å] | 9.500 | 9.500 |
| Rmerge | 0.142 | 0.549 |
| Number of reflections | 26821 | |
| <I/σ(I)> | 44.8 | 2.5 |
| Completeness [%] | 92.1 | 71.8 |
| Redundancy | 25.1 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | MPD, ethanol, magnesium chloride, ammonium chloride, spermine, spermidine, MES , pH 6.5, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | magnesium chloride | ||
| 11 | 1 | 2 | ammonium chloride | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | ethanol | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | ammonium chloride | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 1 | spermidine | ||
| 7 | 1 | 1 | MES | ||
| 8 | 1 | 1 | H2O | ||
| 9 | 1 | 2 | MPD |
