4V45
E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10-21 |
| Detector | RIGAKU |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.500, 207.200, 510.200 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 68.500 - 2.600 |
| R-factor | 0.23 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1F49 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.618 |
| Data reduction software | WEIS |
| Data scaling software | CCP4 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.500 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.360 |
| Number of reflections | 523624 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 70.0 | |
| Redundancy | 1.9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 295 | Cacodylate, PEG 8000, MgSO4, NaCl, BME, pH 5.9, VAPOR DIFFUSION, HANGING DROP at 295K, pH 5.90 |
